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. 2018 Apr 23;9:1588. doi: 10.1038/s41467-018-04035-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — RT probe reacts with both Cys and GSH in physiological conditions. a Reaction of 200 μM of GSH with RT probe. In our experience, such concentrations of GSH are often found in various biological specimen, such as tissues or cells. GS-RT separation resulted in three almost symmetrical peaks at 4.209, 4.464, and 4.768 min of retention time, respectively. b Reaction of 200 μM of Cys with RT probe. This Cys concentration is often found in various biological samples. The separation of Cys-RT resulted in one well-resolved symmetrical peak at 4.848 min retention time, with a response in terms of fluorescence intensity higher than those of GS-RT. c A representative chromatogram of thiols determination in mouse hippocampus cells. In these conditions, the levels of Cys (3.670 min retention time) and GSH (3.928 min retention time) are almost identical (110 and 125 μM, respectively). d Chromatogram of thiols determination in H₂O₂-treated HeLa cells. Levels of Cys and GSH are similar (20 and 28 μM, respectively)