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. 2018 Apr 23;8:6419. doi: 10.1038/s41598-018-24914-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Maximum Likelihood phylogenetic tree of fungal and animal HECT sequences. The best ML value was obtained with the LG + R10 model of sequence evolution and perturbation strength = 0.5. Numbers indicate ultrafast bootstrap support. Blue: fungal groups. In all cases but HUL4A, they correspond to the fungal HECT subfamilies shown in Fig. 2. HUL4A subfamily sequences are here divided into two groups. Red: animal subfamilies; these groups include a sequence of the choanoflagellate Monosiga brevicollis. Black: animal subfamilies for which no orthologous choanoflagellate sequences were found.