Table 1.
gRNAs
| Target | gRNA name | gRNA sequence (20xN-NGG) |
|---|---|---|
| p53 Exon3 | p53 gRNA #1 (759) | AGTGAAGCCCTCCGAGTGTC-AGG |
| p53 Exon3 | p53 gRNA #2 (760) | AGGAGCTCCTGACACTCGGA-GGG |
| p53 Exon3 | p53 gRNA #3 (761) | GACACTCGGAGGGCTTCACT-TGG |
| p63 Exon3 | p63 gRNA #1 | TCCACAAAGTTCAACTCGAT-GGG |
| p63 Exon4 | p63 gRNA #2 | CCGTCACGCTATTCTGTGCG-TGG |
| p63 Exon4 | p63 gRNA #3 | AGCCCCAGGTTCGTGTACTG-TGG |
| p73 Exon3 | p73 gRNA #1 | CCGGGGTAGTCGGTATTGGA-AGG |
| p73 Exon3 | p73 gRNA #2 | CGGGGTGTAGGGGCTCGCCG-GGG |
| Puma Exon1 | Puma gRNA #1 | ATGGCCCGCGCACGCCAGGA-GGG |
| Puma Exon1 | Puma gRNA #2 | AGCTCTCCGGAGCCCGTAGA-GGG |
| Puma Exon1 | Puma gRNA #3 | GGAAGGGGCGCGGACTGTCG-CGG |
| Bim Exon1 | Bim gRNA #1 | ACTTACATCAGAAGGTTGCT-TGG |
| Bim Exon1 | Bim gRNA #2 | TTGCGGTTCTGTCTGTAGGG-AGG |
| Foxo1 Exon1 | Foxo1 gRNA #1 | TCGTCGCGCCGCAACGCGTG-GGG |
| Foxo1 Exon1 | Foxo1 gRNA #2 | GGAGAGTGAGGACTTCGCGC-GGG |
| Foxo3a Exon1 | Foxo3a gRNA #1 | CACGCCGCCACCGATCACCA-TGG |
| Foxo3a Exon1 | Foxo3a gRNA #2 | TCTCGATGGCGCGGGTGATC-AGG |
| Luciferase | Luciferase gRNA | ACCGCTCCGGCGAAGGCGAA-NGG |