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. 2018 Apr 23;8:6394. doi: 10.1038/s41598-018-24748-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Design and illustration of the microfluidic device. (A) A schematic diagram of the device for studying bacterial chemotaxis mechanism. Microfluidic device comprised of four separated cell culture chambers, one central bacteria channel and micro-channels between them. (B) The analysis region was sited between two chambers of cell culture, where the preferential accumulation of bacteria could be quantified. Micro-channels were the barriers between the central bacteria channel and the cell chambers, and also acted as flow regulators. (C) A photographic overview of the integrated microfluidic device.