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. 2018 Apr 23;8:6394. doi: 10.1038/s41598-018-24748-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Verification of the possible biochemical candidates. (A) ELISA quantification of the possible biochemical candidates’ expression in normal and cancer cells revealed that NCI-H460 expressed more CLU, SRGN and TGFβ2 than 16HBE. (B) Quantitation of bacterial chemotaxis of the possible candidates. CLU, SRGN and TGFβ2 were tested as biochemical candidates that attracted E. coli towards NCI-H460. Clear preference was observed for CLU against control, but no significant chemotaxis was noticed for SRGN and TGFβ2. The n = 9 represents 9 biological replicates, the error bars represent the standard deviation of means, and a difference of P < 0.05 was considered statistically significant.