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. 2018 Apr 17;9:729. doi: 10.3389/fimmu.2018.00729

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Copyright © 2018 Pouw, Brouwer, van Beek, Józsi, Wouters and Kuijpers.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Sucrose gradients of normal human serum (NHS) and CFHR3-deficient serum. Western Blots of immunoprecipitation (IP) of fractions (1–24) from a sucrose gradient with (A) NHS or (B) a serum pool deficient for FHR-3 (CFHR3^−/−). IP was performed using anti-FHR-3.3, Western Blots were stained with biotinylated polyclonal anti-FHR-3 antibody. Fractions containing IgM (970 kDa, fractions 3–7), IgG (150 kDa, fractions 15–17), or albumin (66 kDa, fractions 17–21) are indicated above the blots and used as size reference. Identified proteins are indicated with arrowheads on the right side.