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. 2018 Apr 17;9:729. doi: 10.3389/fimmu.2018.00729

Figure 4.

Figure 4

Development of the factor H-related (FHR)-4A specific ELISA. (A) Representative result on the reactivity of the FHR-4A ELISA against rhFHR proteins and plasma-derived FH. Anti-FHR-4A.04 was used as monospecific catching mAb, and binding of antigen was detected using cross-reactive polyclonal anti-FHR-3. (B) Calibration of pooled normal human serum using rhFHR-4A (10 µg/mL) in the FHR-4A ELISA. (C) Comparison of FHR-4A levels in seven paired serum and EDTA plasma samples using the FHR-4A ELISA. Each point represents the mean of three independent measurements per sample. (D) Effect of multiple freeze/thaw cycles. Each point represents the mean of three independent measurements per sample with error bars indicating SD. Dashed lines indicate 90–110% range. (E) Concentration of FHR-4A in 129 healthy donor sera, measured by ELISA. Each point represents the mean of three independent measurements per serum sample. Line indicates mean with SD. (F) Scatter plot of FHR-4A levels versus CRP levels determined in 78 patients with acute bacterial infection. Correlation was assessed using Spearman’s correlation test.