Fig. 3. Expression of the necroptosis regulator RIPK1 correlates with inhibition of autophagy and lysosomal function after SCI.

a The time course of RIPK1 protein expression in spinal cord tissue surrounding injury site following SCI in mice. Full unedited western blots are presented in Supplemental Figure S1c. b Quantification of RIPK1 data from a and S1c. n = 5. c IHC staining demonstrates accumulation of RIPK1 and p62/SQSTM1 in the same ventral horn cells at 24 h after SCI in mice. Images were acquired at ×20 magnification. Full time course images for RIPK1 are presented in Supplemental Figure S4a. d Quantification of RIPK1 data in c and S4a. n = 3–5. e Quantification of RIPK1 and SQSTM1 data in c. At day 1 after SCI 47.8% of RIPK1 positive cells were also positive for p62/SQSTM1. n = 8–13. All data are presented as mean±SE. *p < 0.05, **p < 0.01, ***p < 0.001