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. 2018 Apr 23;8:6392. doi: 10.1038/s41598-018-24646-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Microscopic immunofluorescent images of labelled sprouting HUVECs, in Matrigel enriched media, on top of gelatin coated glass (NoPrint, top row). Sprouting HUVECS in Matrigel enriched media on top of a line pattern of fluorescent IgG beads coated with gelatin (BeadOnly, middle row) or on a Dll4 ligand immobilized with fluorescent IgG beads line pattern coated with gelatin (BeadDll4, bottom row). First three columns contain intensity values separately. The last column contains the merged image with in green; actin stained with phalloidin, in pink; fluorescent IgG beads, and in blue; nuclei stained with DAPI. Scale bar represents 250 µm.