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. 2018 Apr 17;9:605. doi: 10.3389/fimmu.2018.00605

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Copyright © 2018 Rohde, Schusser, Hron, Farkašová, Plachý, Härtle, Hejnar, Elleder and Kaspers.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Alignment of tumor necrosis factor (TNF)-α amino acid sequences from chicken and other vertebrates. The positions of predicted protein domains and of the TNF superfamily motif are indicated above the alignment. The transmembrane domain of the chicken protein was predicted using the THMM 2.0 server (http://www.cbs.dtu.dk/services/TMHMM/). The GenBank accession numbers of the TNF-α proteins are the following: green anole (Anolis carolinensis; XP_008103955), king cobra (Ophiophagus hannah; ETE57607), American alligator (Alligator mississippiensis; XP_006258274), painted turtle (Chrysemys picta bellii; XP_008175031), African clawed frog (Xenopus tropicalis; NP_001107143), human (Homo sapiens; AAA61198), and house mouse (Mus musculus; AAB65593).