Table II.
Results of metabolic experiment of BR1-5 biosynthetic intermediates in Arabidopsis wild type and bri1-5
| Substrate | Metabolite
|
|
|---|---|---|
| Wild type (Ws-2) | bri1-5 | |
| [2H6]CN | [2H6]6-DeoxoCT (1) | [2H6]6-DeoxoCT (3) |
| [2H6]6-DeoxoCT | [2H6]6-DeoxoTE (0.5), [2H6]6-DeoxoTY (1) | [2H6]6-DeoxoTE (0.5), [2H6]6-DeoxoTY (15) |
| [2H6]6-DeoxoTE | [2H6]6-Deoxo3DT (10), [2H6]6-DeoxoTY (14), [2H6]6-DeoxoCS (0.5), [2H6]TY (0.1) | [2H6]6-Deoxo3DT (3), [2H6]6-DeoxoTY (45), [2H6]6-DeoxoCS (3), [2H6]TY (0.2), [2H6]CS (3) |
| [2H6]6-Deoxo3DT | [2H6]6-DeoxoTE (390), [2H6]6-DeoxoTY (2600), [2H6]6-deoxoCS (26), [2H6]TY (4), [2H6]CS (2) | [2H6]6-DeoxoTE (100), [2H6]6-DeoxoTY (1000), [2H6]6-DeoxoCS (4), [2H6]TY (15), [2H6]CS (15) |
| [2H6]6-DeoxoTY | [2H6]6-DeoxoTE (5), [2H6]6-Deoxo3DT (20), [2H6]6-DeoxoCS (2), [2H6]TY (0.5), [2H6]CS (2) | [2H6]6-DeoxoTE (15), [2H6]6-Deoxo3DT (26),[2H6]6-DeoxoCS (28), [2H6]TY (80), [2H6]CS (28) |
| [2H6]6-DeoxoCS | [2H6]CS (8) | [2H6]CS (10) |
| [2H6]6-OH CS | [2H6]CS (4) | [2H6]CS (20) |
| [2H6]TE | [2H6]TY (50), [2H6]CS (1) | [2H6]TY (100), [2H6]CS (3) |
| [2H6]3DT | [2H6]TE (10), [2H6]TY (500) | [2H6]TE (2), [2H6]TY (66) |
| [2H6]TY | – | [2H6]CS (1) |
| [2H6]CS | – | [2H6]BL (5) |
Values in parentheses indicate the detected amount (nanograms) of each metabolite.