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. 2018 Jan 20;69(9):2355–2365. doi: 10.1093/jxb/ery014

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© The Author(s) 2018. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 1. — Phylogenetic analysis and sequence alignment of D14 orthologs from monocots and dicots. (A) Phylogenetic analysis of D14 orthologs from monocots and dicots. The phylogenetic tree was generated with 23 full-length amino acid sequences of D14 orthologs using the Maximum Likelihood method based on the WAG model (100 replicates) in MEGA7 (Whelan and Goldman, 2001; Kumar et al., 2016). The percentage of trees in which the associated taxa clustered together is shown next to the branches. The tree is drawn to scale, with branch lengths measured in the number of substitutions per site. The GenBank accession numbers of D14 orthologs in the presented species are, from top to bottom: Arabidopsis thaliana D14 (NP_566220), Brassica rapa D14 (XP_009130408), Brassica oleracea D14 (XP_013638430), Brassica napus D14 (CDY42894), Punica granatum D14 (OWM70752), Nelumbo nucifera D14 (XP_010248100), Gossypium raimondii D14 (XP_012451974), Hevea brasiliensis D14 (XP_021646820), Populus trichocarpa D14 (XP_002302409), Glycine max D14 (XP_003557012), Medicago truncatula D14 (XP_003589086), Pisum sativum RMS3 (AMB61024), Nicotiana attenuata D14 (XP_019258478), Petunia hybrida DAD2 (AFR68698), Solanum lycopersicum D14 (XP_004238093), Saccharum hybrid D14 (AJY78078), Sorghum bicolor D14 (XP_002468316), Zea mays D14 (NP_001150635), Setaria italica D14 (XP_004985292), Hordeum vulgare D14 (AJP07999), Triticum asetivum D14 (AK332360), Brachypodium distachyon D14 (XP_003558555), and Oryza sativa D14 (XP_015631400). (B) Sequence alignment and structural annotation of D14 orthologs. ESPript was used to analyze the multiple sequence alignments generated by Clustal Omega (Sievers et al., 2011; Robert and Gouet, 2014) with the D14 orthologs listed in (A). Secondary structure elements of the rice D14 crystal structure (PDB code: 4IH9) are displayed on top of the alignments. Identical and conserved residues are highlighted by red and yellow backgrounds, respectively. The three catalytic residues, Ser, Asp, and His, are indicated by green stars.