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. 2018 Jan 20;69(9):2355–2365. doi: 10.1093/jxb/ery014

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© The Author(s) 2018. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 2. — Rice D14 rescues the Arabidopsis d14 mutant. (A and B) Rice D14 rescued the branching phenotype of Atd14-1. (A) Representative branching phenotypes of 7-week-old Col-0, Atd14-1, and four independent T₃ transgenic lines AtD14pro:D14ΔN L2 (line 2), L3, L5, and L6 in the Atd14-1 background; scale bars=1 cm. (B) Quantitative analysis of primary rosette branches of the indicated plants; data are means ±SD (n=20). Error bars indicate the SD; bars with the same letter are not significantly different from one another (ANOVA+Tukey HSD, P<0.01). (C) RT–PCR analysis of the AtD14 or D14 transcript levels in the indicated plants described in (A). The Arabidopsis ACTIN1 was used as an internal control. (D and E) Rice D14 rescued the hypocotyl phenotype of Atd14-1. (D) Representative hypocotyl phenotypes of 7-day-old Col-0, Atd14-1, and AtD14pro:D14ΔN L3 (T₃) seedlings; scale bars=5 mm. (E) Relative hypocotyl lengths of the indicated seedlings; data are means ±SD (n=30). Error bars indicate the SD; bars with the same letter are not significantly different from one another (ANOVA+Tukey HSD, P<0.01). (F and G) Rice D14 rescued the leaf phenotype of Atd14-1. (F) Representative leaf phenotypes of 4-week-old Col-0, Atd14-1, and AtD14pro:D14ΔN L3 (T₃); scale bars=1 cm. (G) Quantitative analysis on the leaf length/leaf width ratio for the sixth leaves of the indicated plants; data are means ±SD (n=20). Error bars indicate the SD; bars with the same letter are not significantly different from one another (ANOVA+Tukey HSD, P<0.01).