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. 2018 Jan 20;69(9):2355–2365. doi: 10.1093/jxb/ery014

Fig. 3.

Fig. 3.

Rice D14 physically interacts with the Arabidopsis SL signaling components. (A) Rice D14 efficiently bound Arabidopsis MAX2 in the presence of rac-GR24. Pull-down assay using recombinant His6-MAX2 and GST–D14 or GST–AtD14 in the absence or presence of rac-GR24. GST-fused proteins were detected by anti-GST antibody and the PVDF membrane was stained with MemStain to show equal loading. (B) Rice D14 efficiently bound Arabidopsis SMXL6 in the presence of rac-GR24. Pull-down assay using recombinant Flag-SMXL6 and GST–D14 or GST–AtD14 in the absence or presence of rac-GR24. GST-fused proteins were detected by anti-GST antibody and the PVDF membrane was stained with MemStain to show equal loading.