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. 2017 Dec 23;69(9):2305–2318. doi: 10.1093/jxb/erx428

Fig. 2.

Fig. 2.

Liquid chromatography–tandem mass spectrometry analysis of sorghum root exudates. The total ion current chromatograms were integrations of multiple reaction monitoring chromatograms with transitions selected for strigol, sorgomol, 5-deoxystrigol (5-DS), and carlactone (CL). (A) Aquaculture filtrate of sorghum grown under phosphate-deficient conditions. (B) Aquaculture filtrate of sorghum grown under phosphate-deficient conditions and supplemented with fluridone. (C) Conversion of CL (1) to 5-DS (9) and sorgomol (10). (D) Conversion of carlactonoic acid (CLA) (2) to 5-DS (9) and sorgomol (10). (E) Conversion of 5-DS (9) to sorgomol (10). IS, internal standard.