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. 2017 Dec 23;69(9):2305–2318. doi: 10.1093/jxb/erx428

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© The Author(s) 2017. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 4. — Liquid chromatography–tandem mass spectrometry analysis of Tonko cotton root exudates. The total ion current chromatograms were integrations of multiple reaction monitoring chromatograms with transitions selected for strigol, strigyl acetate, sorgomol, 5-deoxystrigol (5-DS), and carlactone (CL). (A) Aquaculture filtrate of cotton grown under phosphate-deficient conditions. (B) Aquaculture filtrate of cotton grown under phosphate-deficient conditions and supplemented with fluridone. (C) Conversion of CL (1) to 5-DS (9), strigol (7), and strigyl acetate (11). (D) Conversion of carlactonoic acid (CLA) (2) to 5-DS (9), strigol (7), and strigyl acetate (11). (E) Conversion of 5-DS (9) to strigol (7) and strigyl acetate (11). IS, internal standard.