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. 2017 Dec 23;69(9):2305–2318. doi: 10.1093/jxb/erx428

Fig. 5.

Fig. 5.

Liquid chromatography–tandem mass spectrometry analysis of moonseed root exudates. The total ion current chromatograms were integrations of multiple reaction monitoring chromatograms with transitions selected for strigol, strigyl acetate, 5-deoxystrigol (5-DS), and carlactone (CL). (A) Root culture filtrate of moonseed grown under phosphate-deficient conditions. (B) Root culture filtrate of moonseed grown under phosphate-deficient conditions and supplemented with fluridone. (C) Conversion of CL (1) to strigol (7). (D) Conversion of carlactonoic acid (CLA) (2) to strigol (7). (E) 5-DS (9) was not converted to strigol (7). IS, internal standard.