Table 1.
Conditions used for the detection of strigolactones with liquid chromatography–tandem mass spectrometry
| Strigolactone | Scan mode |
Transition (m/z) |
Cone voltage (V) |
Collision energy (eV) |
|---|---|---|---|---|
| CL (1) | + | 303.1>97.0 | 30 | 18 |
| 19-OH-CL | + | 301.2>185.1 | 30 | 18 |
| CLA (2) | − | 331.2>113.0 | 30 | 18 |
| MeCLA (3) | + | 347.2>97.0 | 35 | 20 |
| 4-DO (4), 5-DS (9) | + | 331.2>216.1 | 28 | 18 |
| + | 331.2>97.0 | 28 | 18 | |
| Orobanchol (5) | + | 347.1>233.1 | 32 | 14 |
| + | 347.1>205.1 | 32 | 14 | |
| + | 347.1>97.0 | 32 | 14 | |
| Heliolactone (6) | + | 361.2>233.1 | 35 | 18 |
| + | 361.2>97.0 | 35 | 18 | |
| Strigol (7), epi-strigol (IS) | + | 369.1>272.1 | 38 | 20 |
| + | 329.1>97.0 | 30 | 20 | |
| Orobanchyl acetate (alectrol) (8) | + | 411.1>254.1 | 42 | 20 |
| + | 389.2>233.1 | 40 | 18 | |
| Sorgomol (10) | + | 364.1>317.1 | 15 | 16 |
| + | 317.1>97.0 | 30 | 20 | |
| Strigyl acetate (11) | + | 411.2>254.1 | 45 | 20 |
| + | 406.1>215.1 | 20 | 18 |
Numbers in bold for the strigolactones refer to Fig. 1. IS, internal standard.