Table 3:
Differentially expressed membrane protein families in MtzR lines, including subgroups and protein and annotation features along with overall differential expression (DE) trends in MtzR lines
| Membrane association | ||||||
|---|---|---|---|---|---|---|
| Group | Subgroup | TMHa | Extra/Intracellular domains | Additional features | DE trends | Key DE ORFs |
| EGF-like proteins | VSP | Yes | CXXC-rich extracellular | S-Palmitoyl necessary for lipid raft localization and lipid signaling [74] | Large DE in terms of proportion and magnitude, little specific variant overlap and variable directionality | GL50803_137 620 |
| CRGKA cytoplasmic tail | Citrullination of arginine [102] | GL50803_37 093 | ||||
| HCMP | Yes | CXC/CXXC extracellular | Possible organelle membrane localization. | Several highly upregulated/downregulated variants, little specific variant overlap | GL50803_112 673 | |
| GL50803_112 633 | ||||||
| Tenascin/Notch-like | Yes | EGF-like conserved site (IPR013032); usually extracellular | Some possess IPR013111 (EGF-like domain, extracellular) | DE with varying directionality between lines | GL50803_11 420 | |
| GL50803_16 322 | ||||||
| Peptidases | Cysteine-rich | Yes* | Growth factor receptor cysteine-rich domain (IPR009030) | Separate from VSP/HCMP, do not contain IPR005127 or IPR006212 annotations | Downregulation | GL50803_14 225 |
| GL50803_101 832 | ||||||
| Dipeptidyl-peptidases | Yes* | Serine-type (GO: 0 008236), cysteine-type (GO:0 008234) or dipeptidyl-peptidase activity (GO:0 008239) | Can localize to plasma membrane in absence of TMH [49] | Dipeptidyl-peptidase III upregulation [106] | GL50803_15 574 | |
| Possible role in signal transduction, particularly during encystation [49] | Alanyl dipeptidyl peptidase downregulation (713, WB) | |||||
| Dipeptidyl-peptidase I downregulation (WB). | ||||||
| ABC transporters | Plasma membrane | Yes | IPR017871 (ABC transporter, conserved site) | Features some lipid-transporting ATPases | DE with varying directionality between lines | GL50803_115 052 |
| GL50803_16 592 | ||||||
| Giardins | Alpha-Giardins | No | Calcium-depending phospholipid binding (GO:0 005544) | Localize to membrane and cytoskeletal structures, including as flagellar [52, 103–105] | Downregulation | |
| Evidence for dual acylation [106] | ||||||
| Beta-Giardin | No | Cytoskeletal | Ventral disc localization [53] | Downregulation | GL50803_4812 | |
ORFs listed in the last column were observed to be differentially expressed in at least 2/3 MtzR lines. *Some, but not all, members possess annotated TMH.