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IEF gel showing PME by ruthenium red staining. Seed parts were extracted after their excision from germinated seeds having radicle lengths of 15 mm. Equal amounts of protein (15 μg) in crude extracts from different seed parts were loaded in each lane. MM, Micropylar end of the megagametophyte; CM, chalazal end of the megagametophyte; R, radicle; C, cotyledons.
