Figure 3.
Effects of temperature, pH, and cations on PME activity. Data are the average of three replicates ± se. A, Thermostability of PME. Partially purified PME extracts were maintained for 5 min at the temperatures indicated and then assayed for activity. B, Effects of pH on PME activity. The activities of partially purified PME extracts were assayed in gels having different pH values. C and D, Effects of Na+ (C) or Ca2+ (D) on PME activity. The activities of the partially purified PME extracts were determined using gels containing different concentrations of NaCl (C) or CaCl2 (D).