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. 2018 Apr 23;15:74. doi: 10.1186/s12985-018-0988-5

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Determine the sensitivity of detection of B19V, CMV and HSV-1/2 by qPCR. a: A high coefficient of correlation (r2 = 0.9975) between the amplification cycle number (Ct values) and copy number representing the B19V virus titer. The standard curve indicates that qPCR can be used effectively to evaluate even low level of B19V DNA in patients. b: A high coefficient of correlation (r2 = 0.9946) between the amplification cycle number (Ct values) and copy number representing the CMV virus titer. The standard curve indicates that qPCR can be used effectively to evaluate even low level of CMV DNA in patients. c: A high coefficient of correlation (r2 = 0.9964) between the amplification cycle number (Ct values) and copy number representing the HSV-1/2 virus titer. The standard curve indicates that qPCR can be used effectively to evaluate even low level of HSV-1/2 DNA in patients