Table 2.

The test of repeatability based on SYBR Green PCR system

a. The serially diluted B19V standards of 1 × 101–106 copies per ul to measure the intra and inter repeatability
	Plasmid copies(cp/μl)	B19. Ct (‾x ± s)	B19. CV (%)
Inter Repeatability	1 × 106	15.91 ± 0.07	0.46
	1 × 105	19.60 ± 0.08	0.41
	1 × 104	23.71 ± 0.05	0.21
	1 × 103	28.12 ± 0.25	0.91
	1 × 102	31.37 ± 0.20	0.64
	1 × 101	35.45 ± 0.63	1.80
Intra Repeatability	1 × 106	15.46 ± 0.66	4.80
	1 × 105	19.86 ± 0.53	3.38
	1 × 104	23.86 ± 0.23	1.04
	1 × 103	27.99 ± 0.25	0.66
	1 × 102	31.42 ± 0.17	0.41
	1 × 101	35.21 ± 0.86	2.85
b. The serially diluted CMV standards of 1 × 101–106 copies per ul to measure the intra and inter repeatability
	Plasmid copies(cp/μl)	CMV. Ct (‾x ± s)	CMV. CV (%)
Inter Repeatability	1 × 106	15.52 ± 0.16	1.03
	1 × 105	20.27 ± 0.5	2.50
	1 × 104	24.02 ± 0.69	2.87
	1 × 103	27.83 ± 0.15	0.52
	1 × 102	31.65 ± 0.61	1.94
	1 × 101	35.24 ± 0.23	0.64
Intra Repeatability	1 × 106	15.80 ± 0.43	2.93
	1 × 105	20.72 ± 0.71	3.10
	1 × 104	24.43 ± 0.75	2.47
	1 × 103	28.26 ± 0.60	2.18
	1 × 102	32.36 ± 1.08	3.21
	1 × 101	35.88 ± 0.79	2.23
c. The serially diluted HSV standards of 1 × 101–106 copies per ul to measure the intra and inter repeatability
	Plasmid copies(cp/μl)	HSV. Ct (‾x ± s)	HSV. CV (%)
Inter Repeatability	1 × 106	16.03 ± 0.16	1.01
	1 × 105	19.67 ± 0.11	0.56
	1 × 104	23.25 ± 0.03	0.11
	1 × 103	26.73 ± 0.18	0.66
	1 × 102	30.69 ± 0.52	1.70
	1 × 101	34.52 ± 1.06	3.07
Intra Repeatability	1 × 106	16.73 ± 0.68	4.32
	1 × 105	20.31 ± 0.63	3.20
	1 × 104	24.02 ± 0.74	3.26
	1 × 103	27.51 ± 0.75	2.90
	1 × 102	31.48 ± 0.85	2.54
	1 × 101	35.84 ± 1.22	3.17

Ct Cycle threshold, CV Coefficient of Variation