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Amino acid distances of 157 EIV HA sequences between the sublineages of EIVs, including the seven complete HA sequences from the present study. Distances were calculated using the Poisson correction model40 in MEGA 7.33
| EIV groups | Distance (%) | Standard error |
|---|---|---|
| Pre-divergence/Brazil 2015 | 6.29 | 0.009 |
| Brazil 2015/Florida I | 1.40 | 0.003 |
| Brazil 2015/Florida II | 3.07 | 0.006 |
| Brazil 2015/American | 3.85 | 0.007 |
| Brazil 2015/Eurasian | 4.40 | 0.008 |
| Florida II/Florida I | 2.37 | 0.004 |
