Table 1.
Comparison of the effectiveness of different treatments in the three types of biofilms on SS and PP surfaces.
| Disinfectant | Concentration (ppm) | Temperature (°C) | Time exposure (min) | Log10 CFU/cm2 reduce on SS ± DSa |
Log10 CFU/cm2 reduce on PP ± DSa |
||||
|---|---|---|---|---|---|---|---|---|---|
| S. aureus 4E monospecies biofilms | Salmonella spp. monospecies biofilms | Dual-species biofilms | S. aureus 4E monospecies biofilms | Salmonella spp. monospecies biofilms | Dual-species biofilms | ||||
| PAA | 10 | 25 | 10 | 3.22 ± 0.97 ABbac | 5.86 ± 0.30 ABb | ND | 3.10 ± 0.13 DEFGHa | 3.10 ± 0.69 ABa | ND |
| 10 | 50 | 10 | 5.06 ± 0.51 EFa | 5.70 ± 0.41 ABCa | ND | 2.04 ± 0.06 ABCb | 2.73 ± 0.34 BDEc | 6.14 ± 0.45 ABa | |
| 10 | 25 | 15 | 3.33 ± 0.10 ABa | 5.88 ± 0.23 Ab | 4.86 ± 0.35 Ac | 5.24 ± 0.25 Jc | 2.69 ± 0.37 BDEd | ND | |
| 10 | 50 | 15 | 5.26 ± 0.23 EFa | 5.88 ± 0.25 Ab | ND | 1.67 ± 0.07 ABc | 3.43 ± 0.42 ACd | ND | |
| 3500 | 25 | 10 | ND | ND | ND | ND | ND | ND | |
| 3500 | 50 | 10 | ND | ND | ND | ND | ND | ND | |
| 3500 | 25 | 15 | ND | ND | ND | ND | ND | ND | |
| 3500 | 50 | 15 | ND | ND | ND | ND | ND | ND | |
| NaClO | 100 | 25 | 10 | 1.97 ± 0.09 Ca | 4.91 ± 0.39 BCDEb | 5.07 ± 0.49 ABb | 1.65 ± 0.67 ABa | ND | 6.22 ± 0.50 ABc |
| 100 | 37 | 10 | 3.71 ± 0.38 ABDa | 4.57 ± 0.36 DEFb | 5.52 ± 0.17 Bc | 4.59 ± 0.77 IJabcd | ND | 5.78 ± 0.97 ABbcd | |
| 100 | 25 | 15 | 3.29 ± 0.34 ABa | 4.99 ± 0.81 CDEFb | ND | 2.33 ± 0.88 ABCDEa | ND | ND | |
| 100 | 37 | 15 | 2.95 ± 1.60 ABCa | 4.97 ± 0.30 ABCDbc | 5.62 ± 0.45 Bb | 3.38 ± 0.67 FGHa | ND | 6.67 ± 0.17 Ad | |
| 200 | 25 | 10 | 3.25 ± 0.04 ABa | 4.40 ± 0.23 Fb | ND | 3.24 ± 0.64 EFGHa | ND | ND | |
| 200 | 37 | 10 | 3.39 ± 0.87 ABa | 4.56 ± 0.17 DEFb | 5.64 ± 0.18 Bc | 3.73 ± 0.38 GHIa | ND | ND | |
| 200 | 25 | 15 | 5.34 ± 0.33 Fa | 5.27 ± 0.18 ABCa | ND | 3.90 ± 0.57 HIb | ND | ND | |
| 200 | 37 | 15 | 3.87 ± 0.53 BDa | 5.27 ± 0.51 ABCb | ND | 5.33 ± 0.17 Jb | ND | ND | |
| CB | 100 | 25 | 10 | 1.92 ± 0.13 Ca | 2.10 ± 0.20 Ia | 5.38 ± 0.18 ABb | 1.45 ± 0.17 Ac | 2.20 ± 0.12 EFa | 4.76 ± 0.73 CDb |
| 100 | 50 | 10 | 2.69 ± 0.98 ACa | 4.62 ± 0.17 DEFb | 5.54 ± 0.45 Bc | 2.35 ± 0.72 ABCDEa | 2.41 ± 0.10 DEFa | 4.45 ± 0.49 Db | |
| 100 | 25 | 15 | 2.12 ± 0.24 Ca | 3.48 ± 1.15 GHb | ND | 2.88 ± 0.95 CDEFGabc | 2.50 ± 0.39 DEFa | 4.32 ± 0.42 Dc | |
| 100 | 50 | 15 | 3.23 ± 0.18 ABa | 4.82 ± 0.30 CDEFb | ND | 3.83 ± 0.62 GHIab | 2.81 ± 0.21 BDc | 3.94 ± 0.36 Db | |
| 200 | 25 | 10 | 4.50 ± 0.36 DEa | 4.52 ± 0.08 EFa | ND | 2.62 ± 0.18 BCDEFb | 2.96 ± 0.14 ABDb | 4.49 ± 0.29 Da | |
| 200 | 50 | 10 | 3.68 ± 0.70 ABDa | 2.37 ± 0.05 HIb | ND | 3.76 ± 0.14 GHIa | 2.00 ± 0.07 Fc | 5.61 ± 0.65 BCd | |
| 200 | 25 | 15 | 2.87 ± 0.88 ABCab | 3.43 ± 0.69 Gbc | ND | 2.18 ± 0.15 ABCDa | 2.54 ± 0.24 DEFa | 4.61 ± 0.84 Dc | |
| 200 | 50 | 15 | 3.50 ± 0.42 ABDa | 4.76 ± 0.26 Ib | ND | 2.49 ± 0.57 BCDEFc | 3.98 ± 0.25 Ca | ND | |
a
Mean of three test of population reduction after disinfection in Log10 ± standard deviation.
b
Values in the same column with the same capital letter are not significantly different (p > 0.05).
c
Values in the same row with the same lowercase letter are not significantly different (p > 0.05).
ND: not detected after disinfection treatment.