FIG 2.

EutH promotes S. Typhimurium survival within macrophages. (A) In vitro growth curve of the WT S. Typhimurium (SL1344) and the ΔeutH (CJA052) strains grown in LB broth. Each data point shows the average from three biological replicates. Error bars represent the mean ± standard deviation (SD). (B) Intramacrophage survival and replication of WT (CJA034), ΔeutR (CJA032), or eutR complemented (CJA033) strains in peritoneal exudate macrophages after 7 h postphagocytosis. n = 9 replicates per strain. (C) Intramacrophage survival and replication of WT (AJK61), ΔeutR (CJA023), or ΔeutH (CJA043) S. Typhimurium strains in RAW macrophages after 5 h postphagocytosis. n = 18 replicates per strain. (D) Intramacrophage survival and replication of WT S. Typhimurium (AJK61), ΔeutH (CJA043), and ΔeutH ΔeutR (CJA168) strains after 5 h postphagocytosis in BMDMs. n = 15 replicates per strain. (E) Intramacrophage survival and replication of WT S. Typhimurium (CJA034), ΔeutH (CJA087), and eutH (CJA184) complemented strains after 5 h postphagocytosis in BMDMs. n = 18 replicates per strain. (F) Intramacrophage survival and replication of ΔssaV, ΔssaV ΔeutR (CJA064), and ΔssaV ΔeutH (CJA172) S. Typhimurium strains after 5 h postphagocytosis in BMDMs. ssaV encodes an essential component of the T3SS-2, and the ΔssaV strain is completely defective for T3SS-2-mediated secretion (48). n = 9 replicates per strain. (G) Initial phagocytosis of S. Typhimurium WT (AJK61), ΔeutH (CJA043), and ΔeutH ΔeutR (CJA168) strains at time zero (see Materials and Methods). n = 24 replicates per strain. (H) In vitro growth curve of the S. Typhimurium WT (SL1344) and ΔeutH (CJA052) strains grown in SPI-2 inducing medium. Each data point shows the average from three biological replicates. Error bars represent the mean ± SD. For panels B to G, the mean and standard error of the mean (SEM) are shown. *, P ≤ 0.05; **, P ≤ 0.005; ***, P ≤ 0.0005. P values of >0.05 are not significant (ns).