FIG 4.

EutH enhances S. Typhimurium fitness in the acidified SCV. (A) Intramacrophage survival and replication of the WT (AJK61) or ΔeutH (CJA043) strain in BMDMs without or with concanamycin A treatment. n = 9 replicates per strain per condition. (B) Intramacrophage survival and replication of the WT (AJK61) or ΔeutH (CJA043) strain in BMDMs without or with NH₄Cl. n = 9 replicates per strain per condition. The mean and SEM are shown. *, P ≤ 0.05; **, P ≤ 0.005. P values of >0.05 are not significant (ns).