Contribution of EutH to S. Typhimurium fitness in vivo. (A) Competitive indices of the ΔeutH (CJA046) versus WT (SL1344) strain during colitis. n = 7 or 8 mice. (B) Competitive indices of the ΔeutH (CJA052) versus ΔeutR (CJA007) strain during colitis. n = 8 mice. (C) Competitive indices in the spleen of the ΔeutH (CJA046) versus WT (SL1344) strain, the ΔeutH complemented (CJA192) (eutH+, which contains pGEN::eutS-eutH) versus WT (CJA182, which contains pGEN empty vector) strain, the ΔeutH (CJA052) versus ΔeutR (CJA007) strain, the ΔeutH (CJA043) versus ΔeutB (CJA018) strain, and the ΔssaV ΔeutH (CJA172) versus ΔssaV strain at 6 hpi following intraperitoneal injection. n = 8 to 11 mice per competition. The median and interquartile range are shown *, P ≤ 0.05. P values of >0.05 are not significant (ns).