Drosophila mutants for Tep2 show low DCP-1 expression levels in the midgut following infection with P. luminescens. (A to F) Guts from 7- to 10-day-old tep2 mutants and background control flies (w1118) were stained with DCP-1 (red), DAPI (blue), and phalloidin (green). The dissected, stained tissues of flies injected with 1× PBS (A and B), P. luminescens (Pl) (C and D), and P. asymbiotica (Pa) (E and F) were observed at a ×40 magnification under a confocal microscope. (G) Corrected total cell fluorescence (CTCF) was measured to quantify the expression level of DCP-1 in both background control flies (w1118) and tep2 mutant flies by using ImageJ.