FIG 1.

SavRS is a type II toxin-antitoxin system. (A) Genetic organization of the savRS operon and the location of primers used in the PCR assay. (B) savS and savR are cotranscribed. Gel electrophoresis analysis of PCR products amplified with genomic DNA, reverse-transcribed cDNA with specific primer RT-savS, and RNA. (C) The expression of savR and savS is growth phase dependent. (D) Inhibition of cell growth by SavS. The strain harboring plasmids pET28savS and pLIsavR was induced with 0.5 mM IPTG, and the growth curve was monitored for 12 h. (E) Protein electrophoresis by SDS-PAGE. (F) SavS and SavR form a stable complex in native PAGE.