TABLE 3.

Oligonucleotide primers used in this study

Primer	Oligonucleotide (5′–3′)	Application
savRS-up-F	GTGCAGCGGAATTCGAGCTCGGTACCGCGATTATCAAATGCTTTCG	savRS deletion
savRS-up-R	TGAATATTCAATATCTGAAT	savRS deletion
savRS-down-F	ATTCAGATATTGAATATTCAATAAACCCTCCTTTGTTTTC	savRS deletion
savRS-down-R	TAAAGCTTGCATGCCTGCAGGTCGACCTGTGCTTTAATTGCTTTTC	savRS deletion
savRS-c-F	CCGGAATTCGTCGCTATTTTCCCTACACT	Complementation
savRS-c-R	CGGGGTACCTGTGAACGATAGCATATAGG	Complementation
savS-protein-F	GGAATTCCATATGAGCAATTACACGGTTAAGATTAAA	Protein expression
savS-protein-R	CCGCTCGAGTTAATCATAATGTGACCATGCCGATAG	Protein expression
savR-protein-F	GGAATTCCATATGATTATCACTAGCCCTACAGAAGCG	Protein expression
savR-protein-R	CCGCTCGAGTTAAAGATTATCCCAATCAATATCATCT	Protein expression
PsavRS-F	GAGTTTTTTTAAGCGGATTC	EMSA
PsavRS-R	CTAGTGATAATCATATAAACCCTCC	EMSA
PsavRS-biotin-R	CTAGTGATAATCATATAAACCCTCC	EMSA (5′ biotin)
Phla-F	TTCAACTTTGACTAACCCTC	EMSA
Phla-R	TTTCATCATCCTTCTATTTT	EMSA
Phla-biotin-R	TTTCATCATCCTTCTATTTT	EMSA (5′ biotin)
Pefb-F	ATACCTTCAGACACCAACAT	EMSA
Pefb-R	AATACCTATTGCCGCTAATG	EMSA
Pefb-biotin-R	AATACCTATTGCCGCTAATG	EMSA (5′ biotin)
PsavRS-F120	AACGATAGCATATAGGTATT	DNase I footprinting
PsavRS-FAM-R	CTAGTGATAATCATATAAACCCTCC	DNase I footprinting
UP-L mutant-R	GCAATGCCGCACTTGAGTACGTCAATA	Palindrome mutation
D-L mutant-F	GTACTCAAGTGCGGCATTGCTAATCGTATCGACAGAAAACAAAGGAGGGTT	Palindrome mutation
UP-S mutant-R	TAGCGTACGCCTGTGGACACGTCAATATTACCAACGAG	Palindrome mutation
D-S mutant-F	GTGTCCACAGGCGTACGCTATAATTAGCG	Palindrome mutation
UP-LS mutant-R	GCAATGCCGCCTGTGGACACGTCAATATTACCAACGAG	Palindrome mutation
D-LS mutant-F	GTGTCCACAGGCGGCATTGCTAATCGTATCGACAGAAAACAAAGGAGGGTT	Palindrome mutation
hla-RT-F	AAAGTAGGCTGGAAAGTGAT	qRT-PCR
hla-RT-R	TAGCGAAGTCTGGTGAAA	qRT-PCR
efb-RT-F	GCGGCAATAGGTATTACTAC	qRT-PCR
efb-RT-R	ACCATCATTGTACTCTACGA	qRT-PCR
RT-savS	TTAATCATAATGTGACCATG	Reverse transcription
savS-R	GACCATGCCGATAGTATT	PCR
savS-F	TTAGAGCGATATTCAAGAAGA	PCR
savR-F	GCCCTACAGAAGCGAGAA	PCR
PsavRS-lacZ-F	CCGGAATTCGAGTTTTTTTAAGCGGATTC	pOSsavRS
PsavRS-lacZ-R	CGCGGATCCGGAGGGCTAGTGATAATCAT	pOSsavRS
Phla-lacZ-F	CCGGAATTCTTCAACTTTGACTAACCCTC	pOShla
Phla-lacZ-R	CGCGGATCCGGGACTATACGTGTTTTCATTT	pOShla
Pefb-lacZ-F	CCGGAATTCATACCTTCAGACACCAACAT	pOSefb
Pefb-lacZ-R	CGCGGATCCGGTATCAATTTATTTTTCATGT	pOSefb
efb-up-F	CGGGGTACCTCTATGAGATTATACCTTCAGACAC	His tag insertion
efb-up-R (his)	GTGATGATGATGATGATGTTTAACTAATCCTTGTTTTAATAC	His tag insertion
efb-down-F (his)	CATCATCATCATCATCACTAAAACTTCAATCGTTGCTGTTATC	His tag insertion
efb-down-R	ACGCGTCGACGCACTAGAACTGGAAATGATAA	His tag insertion