FIG 3.

p62 levels increase during virulent C. burnetii infection. hAMs were infected for 2 to 96 h and processed for immunoblot analysis using monoclonal antibodies to detect p62. β-Tubulin served as a loading control, and the blots presented are representative of those for three individual donors and experiments that were used for densitometry analysis. P values were determined using Student's t test. *, P < 0.05; **, P < 0.001. UI, uninfected cells; STV, nutrient-starved uninfected cells (positive control). p62 levels remain stable in cells infected with avirulent bacteria and increase from 72 to 96 hpi in cells infected with virulent C. burnetii.