FIG 6.

p62 phosphorylation at S349 increases during infection, and Nrf2 levels are stabilized. hAMs were infected with C. burnetii NMII for 4 to 96 h and then harvested and assessed by immunoblot analysis to detect phosphorylated p62 (p-p62) and total p62 (A) or Nrf2 (B). β-Tubulin was probed as a loading control, and the blots presented are representative of those from three individual experiments. The levels of phosphorylated p62 increase during infection and Nrf2 levels remain stable, suggesting activation of both proteins.