FIG 1.
Parasitemia, survival, and cytokine responses of BALB/c and C57BL/6 mice during P. c. chabaudi AS infection. BALB/c and C57BL/6 mice were administered with 1 × 106 pRBC, and the parasitemia (A) and survival rate (B) were monitored daily. For evaluation of cytokine production, mice were sacrificed at the indicated time. Serum samples were prepared and total RNA was isolated from spleen. The mRNA level of IFN-γ (C) and IL-10 (D) were measured by real-time PCR. The serum concentration of IFN-γ (E) and IL-10 (F) was determined with ELISA. All data except survival rate are presented as arithmetic means ± SE. Single and double asterisks indicate P values of <0.05 and <0.01, respectively, compared with uninfected mice of the same strain. A pound sign means a P value of <0.05 compared with the other strain at the same time point. The P value for the difference in survival between the two strains was less than 0.01.