FIG 3.

Dynamics of CD19⁺ CD5⁺ CD1d^hi cells and their contribution to IL-10 production during the course of P. c. chabaudi AS infection in C57BL/6 and BALB/c mice. (A) Spleen cells were isolated from mice and stained for membrane CD19, CD5, and CD1d as well as intracellular IL-10. CD19⁺ CD5⁺ CD1d^hi cells were gated as shown in the left and middle panels, and the subgating of IL-10⁺ cells in the CD19⁺ CD5⁺ CD1d^hi population is displayed on the right. (B and C) Change of frequency and absolute number of CD19⁺ CD5⁺ CD1d^hi cells in spleen from C57BL/6 and BALB/c mice. The percentage of IL-10-producing cells in CD19⁺ CD5⁺ CD1d^hi cells (D) and the proportion of CD5⁺ CD1d^hi B cells in CD19⁺ IL-10⁺ cells (E) were also analyzed. (F) Gating strategy used for panel E. Data are presented as arithmetic means ± SE. Single and double asterisks indicate P values of <0.05 and <0.01, respectively, compared with uninfected mice of the same strain. Single and double pound signs mean P values of <0.05 and <0.01, respectively, compared with the other strain at the same time point.