TABLE 1.
Attenuation of gene knockout P. yoelii preerythrocytic stages in BALB/c mice
| Parasite name or genotype | No. of sporozoites used for inoculationa | Mouse | No. of mice patentb | No. of days to patencyc |
|---|---|---|---|---|
| 1971cl1d | 1,000 | BALB/cJ | 3/3 | 3 (3) |
| 1971cl1d | 10,000 | BALB/cJ | 3/3 | 3 (3) |
| lisp2− | 1,000 | BALB/cJ | 6/8 | 5 (4) |
| 7 (2) | ||||
| lisp2− | 10,000 | BALB/cJ | 7/7 | 4 (2) |
| 5 (4) | ||||
| 6 (1) | ||||
| plasmei2− | 50,000 | BALB/cByJ | 0/10 | |
| 200,000 | BALB/cByJ | 3/30 | 5 (2) | |
| 6 (1) | ||||
| 500,000 | BALB/cByJ | 4/30 | 5 (2) | |
| 6 (1) | ||||
| 7 (1) | ||||
| plasmei2− lisp2− | 50,000 | BALB/cJ | 0/30 | |
| 50,000 | BALB/cByJ | 0/10 | ||
| 200,000 | BALB/cByJ | 0/29 | ||
| 500,000 | BALB/cByJ | 0/26 | ||
| fabb/f− | 500,000 | BALB/cJ | 0/10 | |
| 500,000 | BALB/cByJ | 0/20 |
Salivary gland sporozoites were isolated from infected Anopheles stephensi mosquitoes, and mice were i.v. challenged with the listed number of sporozoites.
The number of patent mice per number of mice challenged is indicated. Detection of blood stage patent parasitemia was carried out by Giemsa-stained thin blood smear. Attenuation was considered complete if mice remained blood stage negative for 21 days.
If mice became blood stage patent, the day to patency is listed, with the number of mice that became patent in parentheses.
The marker-free GFP-luciferase-expressing 1971cl1 parasite has a wild-type phenotype in all aspects of the life cycle, including sporozoite infectivity, and was used for the creation of all of the gene knockouts.