FIG 1.

ELISA data showing the secretion of HMGB1 in HGECs and THP-1 stimulated by TNF-α and the effects of anti-HMGB1 antibody on inflammatory cytokines. (A) The supernatants of 10 ng/ml TNF-α-stimulated HGECs and THP-1 were analyzed for secreted HMGB1 using ELISA. Each experiment was performed three times. *, P < 0.05; **, P < 0.01 (one-way ANOVA and Dunnett's test). (B and C) The supernatants of 10 ng/ml TNF-α-stimulated HGECs and THP-1 with or without 50 μg/ml anti-HMGB1 antibody were analyzed by ELISA for levels of released IL-1β in THP-1 (B) and GM-CSF in HGECs (C). Each experiment was performed three times. One-way ANOVA and Tukey-Kramer tests were performed. *, P < 0.05; **, P < 0.01.