TABLE 3.
Primers used for construction of dsbB knockout strains and for verification of dsbB removal
| Primer purpose and primer | Sequence (5′–3′)a |
|---|---|
| Allelic exchange | |
| DsbB_US_Nhe_F | CGCCGCGCTAGCCGGACTGACGGGCTTCAAG |
| DsbB_US_Bgl_R | TTAACGAGATCTTCGCGCCGCTTTTC |
| DsbB_DS_Bgl_F | TGATCGAGATCTAATAGCCCGCATCATTG |
| DsbB_DS_HindIII_R | TCGCCTAAGCTTCGAGATGACCGCCGCCG |
| Knockout confirmation | |
| DsbB_PA_F | TCTGCAGTACGTGAAAAACGAGGA |
| DsbB_PA_R | CCGGGGTTCAACTGCACGTA |
a
Underlined sequences indicate restriction enzyme sites for downstream cloning.