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. 2018 Apr 23;15:120. doi: 10.1186/s12974-018-1146-0

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 9 — TB staining and quantitation of mast cells in the old mouse brains. For the photomicrographs a, b, d, and e, paraformaldehyde-fixed brains from old sham (a, d) and WP-sensitized (b, e) male (a, b) and female (d, e) mice were coronally frozen-sectioned at 40 μm and stained with TB. The lower left insets in the panels a, b, d, and e show the cells indicated with the arrows at a higher magnification. The images in the panels were taken with a ×20 objective (scale bar = 50 μm), whereas the insets were taken with a ×40 objective (scale bar = 20 μm). Quantitative comparisons show the number of granulated (hashed bars) and degranulated (open bars) mast cells observed in male (c) and female (f) brain sections. Values indicate group average ± standard error (n = 4)