FIG 2.

Susceptibilities of representative FKS deletion mutants to cell wall-damaging agents, compared to the parental strain. The spot assay shows comparative growth of representative strains lacking one copy of the essential FKS1 gene or two copies of either FKS2 or FKS3, compared to the Ura⁻ parental strain CAF4-2 and the isogenic Ura⁺ control strain CAF2-1, on control YPD medium and on YPD medium supplemented with Congo red, calcofluor white, or hygromycin B, as indicated. Concentrations of the cell wall-damaging agents are indicated. Strains are indicated on the left. For more details, see the legend to Fig. 1. Note that one of three independent repeats of the spot assay is shown here; see Fig. S4A and B for the two other assays.