TABLE 1.

Pairs of P. falciparum proteins suggested as targets for combinatorial chemotherapy, based on synthetic lethal genetic interactions in S. cerevisiae

Gene 1 identification	Gene 1 producta	Gene 2 identification	Gene 2 product
PF14_0492	Calcineurin subunit B	PFF0170w	Cation/H+ antiporter
PFL0590c	Non-SERCA-type Ca2+-transporting P-ATPase 4	PFF0170w	Cation/H+ antiporter
PFE0485w	Phosphatidylinositol 4-kinase	PFF0305c	Ubiquitin-conjugating enzyme E2
PF08_0031	Dicarboxylate/tricarboxylate carrier	mal_mito_2	Cytochrome c oxidase subunit 1
PFF1105c	Chorismate synthase	PF14_0511	Glucose-6-phosphate dehydrogenase
PFL2465c	Thymidylate kinase	PF13_0176	Apurinic/apyrimidinic endonuclease
MAL13P1.346	DNA repair endonuclease	PF13_0176	Apurinic/apyrimidinic endonuclease
PFB0160w	ERCC1 nucleotide excision repair protein	PF13_0176	Apurinic/apyrimidinic endonuclease
PFF0715c	Endonuclease III homologue	PF13_0176	Apurinic/apyrimidinic endonuclease
PFD0865c	Cdc2-related protein kinase 1	PFF0165c	Conserved Plasmodium protein, unknown function
PFL1635w	Sentrin-specific protease 1	PF10_0092	Metallopeptidase
PF13_0251	DNA topoisomerase 3	PF10_0092	Metallopeptidase
PFF0775w	Pyridoxal kinase-like protein	PFF1025c	Pyridoxine biosynthesis protein
PF11_0192	Histone acetyltransferase	PFF1180w	Anaphase-promoting complex subunit
PFL2440w	DNA repair protein	MAL7P1.94	Prefoldin subunit 3
PF11_0087	DNA repair protein	PF10_0041	U5 small nuclear ribonucleoprotein
PFB0445c	ATP-dependent RNA helicase	PF10_0041	U5 small nuclear ribonucleoprotein
PFE0925c	ATP-dependent RNA helicase	PF10_0041	U5 small nuclear ribonucleoprotein
PF10_0294	Pre-mRNA-splicing factor ATP-dependent RNA helicase	PF10_0041	U5 small nuclear ribonucleoprotein
PFC1060c	U4/U6.U5 tri-small-nuclear-ribonucleoprotein-associated protein 1	PF10_0041	U5 small nuclear ribonucleoprotein
PF13_0096	U4/U6.U5 tri-small-nuclear-ribonucleoprotein-associated protein 2	PF10_0041	U5 small nuclear ribonucleoprotein
PFC0365w	Pre-mRNA-processing factor 19	PF10_0041	U5 small nuclear ribonucleoprotein
PFD0685c	Structural maintenance of chromosomes protein 3	PF10_0041	U5 small nuclear ribonucleoprotein
MAL13P1.214	Phosphoethanolamine N-methyltransferase	PFA0455c	Fatty acid elongation protein, GNS1/SUR4 family
MAL13P1.214	Phosphoethanolamine N-methyltransferase	PFL0950c	Aminophospholipid-transporting P-ATPase
MAL8P1.17	Protein disulfide isomerase	PF10_0092	Metallopeptidase
MAL8P1.17	Protein disulfide isomerase	PFB0920w	DnaJ protein
PF07_0029	Heat shock protein 86	MAL13P1.139	Mitochondrial fission 1 protein
PF07_0029	Heat shock protein 86	PFI0300w	Vacuolar protein sorting-associated protein 46
PF14_0068	rRNA 2′-O-methyltransferase fibrillarin	PFF1180w	Anaphase-promoting complex subunit
PF14_0261	Proliferation-associated protein 2g4	PF14_0612	Zinc finger protein
PF14_0286	NADP-specific glutamate dehydrogenase	PF14_0334	NAD(P)H-dependent glutamate synthase
PF14_0401	tRNA import protein	PF13_0257	Glutamate-tRNA ligase
PFC0510w	E3 ubiquitin-protein ligase	PFI0300w	Vacuolar protein sorting-associated protein 46
PFE0750c	Pre-mRNA-splicing factor	PF14_0688	Pre-mRNA-splicing factor ISY1
PFF1385c	Conserved Plasmodium protein	PFB0920w	DnaJ protein
PFL1140w	Vacuolar iron transporter	PFL0725w	Thioredoxin peroxidase 2

^aSERCA, sarcoendoplasmic reticulum calcium transport ATPase; ERCC1, excision repair cross-complementation group 1.