FIG 5.

Accumulation and release of gepotidacin in human THP-1 monocytes and murine J774 macrophages. (A) THP-1 monocytes were incubated with [¹⁴C]gepotidacin at a fixed concentration (1 mg/liter) and collected (accumulation) (left) or incubated for 30 min and then returned to drug-free medium (efflux) (right). (B) (Left) THP-1 and J774 cells were incubated with [¹⁴C]gepotidacin at different extracellular concentrations for 30 min; (middle) both cell lines were incubated for 30 min with 1 mg/liter [¹⁴C]gepotidacin and collected (accumulation) or returned to drug-free medium for 30 min at the temperatures indicated (efflux); (right) same experiment as in the middle panel, but uninfected and infected THP-1 cells were compared at 37°C. For each graph, the ordinate shows the apparent ratio between the cellular and the extracellular concentrations. Data are means ± SDs (n = 3). Statistical analysis was performed by analysis of variance with the Tukey-Kramer multiple-comparison t test (B, left) and an unpaired multiple t test (B, middle and right).