FIG 6.

Fractionation of cytoplasmic extracts of J774 macrophages by isopycnic centrifugation in a linear sucrose gradient (collected in 12 discrete fractions). Cells were incubated with 1 mg/liter [¹⁴C]gepotidacin for 30 min prior to collection. Results are presented as histograms of the density distribution of [¹⁴C]gepotidacin and of the marker enzymes (lactate dehydrogenase, cytosol; cytochrome c-oxidase, mitochondria; N-acetyl-β-hexosaminidase, lysosomes). The abscissa is the density span of the gradient. The ordinate is the frequency of the distribution, defined as the fractional amount of activity recovered in each fraction divided by the density interval of that fraction. The surface of each section of the diagrams therefore represents the fraction of each constituent recovered in the corresponding density span. Data are from a single experiment that was repeated with very similar results.