FIG 7.

Subcellular distribution of [¹⁴C]gepotidacin, marker enzymes (lactate dehydrogenase [LDH], cytosol; cytochrome c-oxidase [CYTOX], mitochondria; N-acetyl-β-hexosaminidase [NAB], lysosomes) and bacteria in homogenates of THP-1 (left) or J774 (right) cells, expressed as a percentage of the total recovered amount or activity. Cells were infected for 1 h and returned to fresh medium for 2 h to allow complete internalization of the bacteria and exposed to 0.1 mg/liter of [¹⁴C]gepotidacin for 30 min prior to collection. Homogenates were separated into 3 fractions by centrifugation at increasing centrifugal fields (for the main cytological content, i.e., nuclei and unbroken cells, organelles [mitochondria, lysosomes], and the final supernatant). Data are the means from 2 experiments with similar results.