TABLE 2.
Molecular characteristics of selected challenge strain reisolates and transconjugants
| Species or serovar | Designationa | Sequence type | Inc designation(s) | % sequence identityb to pRH-1238 (size of pRH-1238 progeny [kbc]) | Presence of blaNDM-1 and blaCMY-16 | Additional resistance gene(s) present |
|---|---|---|---|---|---|---|
| S. Corvallis | G1-11d-T10 | ST-1541 | IncHI2, IncA/C2, ColpVC | 66.03 (∼100) | Only blaNDM-1 | blaTEM-1B |
| S. Corvallis | G1-16d-T10 | ST-1541 | IncHI2, IncA/C2, ColRNAI | 99.40 (∼180) | Both | |
| S. Corvallis | G1-21d-T10 | ST-1541 | IncHI2, IncA/C2, ColpVC | 99.66 (∼180) | Both | blaTEM-1B |
| S. Corvallis | G1-28d-T10 | ST-1541 | IncHI2, IncA/C2, ColpVC | 95.55 (∼170) | Both | blaTEM-1B |
| S. Corvallis | G1-28d-T10d | ST-1541 | IncHI2, IncA/C2, ColRNAI | 98.69 (∼180) | Both | |
| S. Corvallis | G2-8d-T1 | ST-1541 | IncHI2, IncA/C2, ColpVC, ColRNAI | 79.17 (∼140) | Both | |
| S. Corvallis | G2-10d-T1 | ST-1541 | IncHI2, IncA/C2, ColRNAI | 99.54 (∼180) | Both | |
| S. Corvallis | G2-12d-T1 | ST-1541 | IncHI2, IncA/C2, ColpVC, ColRNAI | 73.46 (∼130) | Both | |
| S. Corvallis | G2-16d-T1 | ST-1541 | IncA/C2, ColRNAI | 95.25 (∼170) | Both | |
| S. Corvallis | G2-25d-T1 | ST-1541 | IncHI2, IncA/C2 | 99.66 (∼180) | Both | |
| S. Corvallis | G2-28d-T1 | ST-1541 | IncHI2, IncA/C2 | 99.54 (>400) | Both | |
| S. Corvallis | G3-16d-T3 | ST-1541 | IncHI2, IncA/C2, ColRNAI | 75.69 (∼140) | Both | |
| S. Corvallis | G3-28d-T3 | ST-1541 | IncHI2, IncA/C2, ColRNAI | 99.45 (∼180) | Both | |
| S. Corvallis | G3-28d-T3d | ST-1541 | IncHI2, IncA/C2, ColRNAI | 99.65 (∼180) | Both | |
| E. coli | G1-21d-T7 (I) | ST-2040 | IncX1, IncA/C2, ColpVC, ColRNAI | 99.76 (∼180) | Both | qnrS1, blaTEM-1B |
| E. coli | G1-21d-T7 (II) | ST-156 | IncA/C2 | 99.83 (∼180) | Both | tet(B), blaTEM-1B |
| E. coli | G1-21d-T1 | ST-2040 | IncX1, IncA/C2, ColpVC, ColRNAI | 99.76 (∼180) | Both | qnrS1, blaTEM-1B |
| E. coli | G2-21d-T5 | ST-117 | p0111, IncA/C2 | 99.68 (∼180) | Both | |
| E. coli | G2-21d-T9 (I) | ST-2485 | IncA/C2 | 99.85 (∼180) | Both | |
| E. coli | G2-25d-T2 (II) | ST-2485 | IncA/C2 | 98.62 (∼180) | Both | |
| K. pneumoniae | G2-25d-T2 (III) | ST-1106 | Col(MGD2), IncA/C2, ColRNAI | 99.53 (∼180) | Both | oqxA, oqxB, blaSHV-1 |
| E. coli | G3-21d-environment (I) | ST-2040 | IncX1, IncA/C2, ColpVC, ColRNAI | 89.62 (∼170) | Only blaCMY-16 | qnrS1, blaTEM-1B |
| E. coli | G3-21d-T3 | ST-2040 | IncX1, IncA/C2, ColpVC, ColRNAI | 99.74 (∼180) | Both | qnrS1, blaTEM-1B |
| E. coli | G3-21d-T6 (I) | ST-2040 | IncX1, IncA/C2, ColpVC, ColRNAI | 95.31 (∼180) | Both | qnrS1, blaTEM-1B |
| S. Paratyphi B (d-Ta+) | G1-16d-T5 | ST-28 | Incl1 | None | Neither |
a
Designations indicate the group (G1 to G3), day of life (1d to 28d), animal (T1 to T11), and subcolony (I to IV) origin.
b
Based on consensus sequence length (CLC Genomics Workbench [v9.5] software).
c
Based on S1-PFGE restriction.
d
Postmortem (cecal content isolates).