Figure 5.

Schematic illustration of SBEIc precursor encoded by 4.6-kb Sbe1c transcript. DNA sequences corresponding to exons 1 to 14 on wheat genomic Sbe1 (Båga et al., 1999; Rahman et al., 1999) are indicated. Hatched area indicates location of predicted transit peptide and domains 1 and 2 encompass SBEI-like sequences. The location of the four highly conserved regions on (βα)₈ barrels of amylolytic enzymes (Svensson, 1994) are indicated by black boxes, and their sequences are shown below. Highly conserved residues are indicated by asterisks and catalytic residues present only on domain 2 are underlined. SBEIc is aligned with the SBEI-like protein deduced from the wSBEI-D2 cDNA (Rahman et al., 1999) and the wheat 87-kD SBEIb (Repellin et al., 1997).