Fig. 1.

MiR-196a is up-regulated in human BC, especially in ER+ tumor tissues. a The expression levels of miR-196a in 46 paired of BC and adjacent normal tissues were analyzed by qRT-PCR and normalized to U6 expression levels. Student’s t-test was used to analyze the difference between the non-tumor tissues and BC group. ** indicates significant difference at P < 0.01. b The miR-196a expression levels of normal adjacent breast tissues and BC tissues were analyzed in the BC database of the public GEO dataset (GSE40525). ** indicates significant difference at P < 0.01. c The relative miR-196a expression levels of BC tumors were analyzed according to ER status (ER-negative, n = 17; ER-positive, n = 29). Data were presented as mean from three independent experiments with triple replicates per experiment. ** indicates significant difference at P < 0.01. d Different GEO dataset GSE22220 was used to analysis the expression levels of miR-196a in ER-negative or ER-positive tissues. * indicates significant difference at P < 0.05. e, f The Kaplan Meier plotter was used to detect the overall survival (OS) of miR-196a in ER+ and ER- BC patients, respectively