Table 2.
Inhibitory activities of compounds isolated from Matricaria recutita L. on rat lens aldose reductase (RLAR), DPPH radical scavenging, and advanced glycation end products (AGEs).
| Compounds | IC50 (μM)1) | ||
|---|---|---|---|
| RLAR | DPPH | AGEs | |
| Agipenin-7-O-β-D-glucoside (1) | 4.25 ± 0.07d | >25.0 | NI |
| Luteolin-7-O-β-D-glucoside (2) | 1.12 ± 0.02b | 7.24 ± 0.38b | NI |
| Penduletin (3) | NI2) | NI | NI |
| Jaceidin (4) | NI | NI | NI |
| Agipenin-7-O-β-D-glucuronide (5) | 1.16 ± 0.04b | 10.58 ± 0.47bc | NI |
| Luteolin-7-O-β-D-glucuronide (6) | 0.85 ± 0.02a | 8.92 ± 0.21b | 3.39 ± 0.17a |
| 3,5-O-di-caffeoylquinic acid (7) | 0.72 ± 0.02a | 12.34 ± 0.63bc | NI |
| 6-Hydroxyapigenin (8) | NI | NI | NI |
| Apigenin (9) | 1.72 ± 0.04c | 15.63 ± 0.34c | NI |
| Luteolin (10) | 1.42 ± 0.03bc | 11.53 ± 0.38bc | 6.01 ± 0.38b |
| Quercetin3) | 1.21 ± 0.04b | — | — |
| L-Ascorbic acid4) | — | 3.75 ± 0.17a | — |
| Aminoguanidine5) | — | — | 98.69 ± 5.31c |
1)The IC50 values are defined as mean ± relative standard derivation (RSD) of half-maximal inhibitory concentrations obtained from three independent experiments performed in duplicate and the range of the inhibitor concentrations adopted to evaluate IC50 was prepared as follows: (1) RLAR: 1, 5, and 10 μg/mL; (2) DPPH: 15, 30, and 75 μg/mL; and (3) AGEs: 10, 25, and 50 μg/mL. 2)NI: no inhibition. 3)–5)Quercetin, L-ascorbic acid, and aminoguanidine are the positive control for RLAR inhibition, DPPH scavenging, and AGEs inhibition. Values within a column marked with different letters are significantly different from each other (p < 0.05).