Skip to main content
. Author manuscript; available in PMC: 2018 Apr 24.
Published in final edited form as: J Proteome Res. 2017 May 25;16(7):2445–2456. doi: 10.1021/acs.jproteome.7b00061

Figure 4.

Figure 4

Comparison of identification results acquired by HCD-OT vs HCD-IT (i.e., HCD fragments scanned by either Orbitrap or dual-cell Ion-Trap) at different loading amounts and numbers of samples. (A) Protein identification at different loading amounts with N = 3/condition, indicating superior performance of HCD-OT (p < 0.05) at high loading amount. (B) The advantage of confident identification by HCD-OT becomes much more prominent when larger numbers of human cell samples are analyzed (cumulative ID number, which is important for MS1 quantification). As a result, HCD-OT was chosen to assign ID to ion-current quantitative features for large biological cohorts.