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. Author manuscript; available in PMC: 2018 Apr 24.
Published in final edited form as: J Proteome Res. 2017 May 25;16(7):2445–2456. doi: 10.1021/acs.jproteome.7b00061

Figure 5.

Figure 5

High-resolution MS1 measurement with narrow-width extraction improves selectivity, sensitivity and quantitative accuracy and precision. (A) Examples for MS1 ion currents of low-abundance peptides under resolutions of 30 k, 60 k and 120 k, acquired in consecutive scan events and decreasing extraction window widths corresponding to the resolutions in the same LC–MS analysis of a human cell digest. (B) Comparison of quantitative accuracy and precision under different MS1 resolutions for quantification of human proteins (true ratio = 3.0 between the two groups and N = 3/group) that were spiked in E. coli. lysate. Details of the sample set is in Supplemental Methods.